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KMID : 0377619710200030233
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Korean Jungang Medical Journal
1971 Volume.20 No. 3 p.233 ~ p.243
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Studies on the Host Killing of Klebsiella Phage
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Abstract
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The author studied on the host killing of Kiebsiella phage and obtained the following results.
The host bacteria were more killed when multiplicity of infection of phage is large than small and, when the multiplicity of infection is large, an upward-curve appearance occurred on dying of host bacteria by phage at 37°C.
Under the same multiplicity of infection, the bacteria-killing rate of phage was almost similar if bacterial concentration is 109~107/ml, while it became remarkably lower in 106/ml of bacteria.
On preserving the host bacteria, at 37°C and 4°C, to which phase adsorption had considerably progressed at warm temperature, the death rate of bacteria at 4°C was higher than that at 37°C. The bacteria were also remarkably killed even though the mixture of bacteria and phage, about 100 of multiplicity of infection, is kept at 4°C from the first.
The bacteria-killing rate of phage was a little higher if the bacteria- are logarithmic growth phase than resting phase even though adsorption rate of phage to both bacteria was similar.
The heated and formal in-treated bacteria and streptomycin-resistant bacteria were less killed by phage than normal ones.
The salts inhibited a little the host-killing activity of phage.
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